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► 19 F molecular tags and labeling protocols for 19 F NMR studies of proteins are reviewed and contrasted. ► 19 F NMR biosynthetic labeling strategies are presented. ► Experimental challenges (loss of function through labeling, line broadening, assignment ambiguities) are discussed. ► Approaches to the study of protein topology, using 19 F NMR, are presented. ► Current...
►We review two-dimensional inversion methods for NMR data. ► Methods reviewed are applicable to relaxation time or diffusion data. ► Numerical methods for solving Fredholm integral equations of the first kind are given. ► Selection of optimum smoothing for Tikhonov regularisation is discussed. ► Tikhonov regularisation is found to provide the best results.
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